Chitin, a structural polysaccharide, is a key component found in the cell walls of fungi. It is known for its potential to support the immune system and contribute to overall health.

Beta-glucans 1,3 and 1,6 are polysaccharides known for their immunomodulatory properties. These compounds have been studied for their ability to enhance the activity of immune cells, promoting a balanced and responsive immune system. Beta-glucans are recognized for their potential to support immune function and overall well-being.

Melanin, a pigment responsible for color in various organisms, including fungi, contributes to the blend’s uniqueness. Melanin has antioxidant properties and may play a role in protecting cells from oxidative stress.

This proprietary blend harnesses the synergistic effects of Chitin, Beta-glucans, and Melanin, offering a comprehensive approach to immune support and potential antioxidant benefits. As with any supplement, it is advisable to consult with a healthcare professional before incorporating it into your wellness routine.

HMC contains Chitin, which is structured in the cell walls of fungi in the form of the finest microfibrils, fine fibers, forming a spatial network with a large surface area. Thus, in 1 gram of HMC, the surface area of ​​chitinal microfibrils can reach up to 1000 m2, which explains the unique absorption capacity of the chitin used in HMC.

Chitin is a bio sorbent with a broad but selective effect. It is a natural biopolymer that contains nitrogen atoms in its molecule, which has a unique sorption capacity to absorb toxins and heavy metals. However, it does not remove the main bioactive and trace elements and does not interfere with the mineral metabolism.

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Vitamin in the shape of a microdroplet enters the capillaries of the oral mucosa and the blood.

The active substance is immediately absorbed by the circulatory system, which spreads throughout the body.

The gastrointestinal tract is not involved in the process, which often makes it difficult to absorb the vitamin completely.

The microemulsion dosage form of the vitamin convincingly guarantees the maximum efficiency of vitamin absorption in the body, up to 95% compared to vitamin D3 in capsules or drops.

In a double-blind, randomized, placebo-controlled in vivo clinical study, SUNSHINE D3 Natural Vitamin Spray was shown to increase vitamin D levels by an average of 123% from baseline within 30 days of study initiation.

Natural vitamin SUNSHINE D3 with high bioavailability contributes to:

• to the normal functioning of the immune system
• for the absorption and normal utilization of calcium and phosphorus
• to keep bones under normal conditions
• to normal muscle function

Natural vitamin SUNSHINE D3

• is naturally highly concentrated vitamin D3
• The spray bottle lasts 125 days or 4 months of use
• can be easily taken with you
• has been tested for accelerated stability
• Does not contain any artificial colors, flavors or gluten

The active ingredient in SUNSHINE D3 is lanolin. Lanolin It is used by almost all vitamin D manufacturers, but its high effectiveness is ensured by its unique spray composition.

In addition to the effects of vitamin D on the skeleton, there is now more evidence that vitamin D supports and promotes immune health, mental health and overall life expectancy.

Ingredients: natural vitamin D3 enriched with orange flavor and natural sweetener xylitol, which supports the health of the oral cavity and teeth.

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Food grade diatomaceous earth COLOFLUSH is an ancient substance and a true modern natural treasure – a safer and simpler solution to our daily needs.

It is composed of the fossilized siliceous skeletons or remains of diatoms. Diatoms are a major group of microscopic algae or unicellular aquatic plants and are among the most common types of phytoplankton. That once flourished as a form of golden brown aquatic algae or plankton that lived in fresh water lakes as a food source for all kinds of aquatic creatures. 

Our COLOFLUSH is cleaned and sterilized in our unique production and combined with baking soda to an alkaline diatomaceous earth for human consumption and contains NO additives. It is a fine, off-white, 10-mim microfine powder that disperses easily in liquid. Contains an average of 96% silica and less than 0.5% crystalline silica. 

The many amazing benefits of FOR LONG LIFE food grade diatomaceous earth:

COLOFLUSH ADVANCED FORMULATED POWDER PLEASE DO NOT CONFUSE WITH INDUSTRIAL SWIMMING POOL SILICATING AGENT THAT HAS BEEN HEAT AND CHEMICALLY TREATED AND INTENDED FOR POOLS AND FISH FILTERS ONLY.

IT IS TOXIC TO LIVING ENTITIES.

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Medical research verified that Bifidobacterium longum has the effect of regulating human intestines and improving health – healthy lifestyle

STREPTOCOCCUS THERMOPHILUS

Can reduce intestinal ph, promote bowels movement to prevent colonization of pathogenic bacteria, secrete bacteriocin to inhibit the growth of pathogenic bacteria

LACTOBACILLUS PARACASEI

It helps promote the balance of microbial flora in the body and stimulate specific and non-specific immune mechanisms, which can prevent certain diseases, enhance physical fitness, and delay aging – healthy lifestyle

LACTOBACILLUS REUTERI

It has strong adhesion to intestinal mucosa to improve the distribution of intestinal flora, antagonize the colonization of harmful bacteria, and avoid suffering from intestinal diseases

LACTOBACILLUS CASEI

It treats intestinal flora disorders and enhances intestinal permeability, improves immunity

LACTOBACILLUS ACIDOPHILUS

Adjusts the balance of the intestinal flora,  helps secrete avidin, and inhibits the proliferation of intestinal microbes

LACTOBACILLUS RHAMNOSUS

Works as a biological barrier of the human intestinal mucosa, regulates intestinal flora, and enhances the body’s immunity

LACTOBACILLUS FERMENTUM

Improves the intestinal environment, regulates intestinal flora, improves immunity

LACTOBACILLUS HELVETICUS

It can adjust the balance of intestinal micro flora and treat diarrhea

LACTOBACILLUS PLANTARUM

With a certain immune regulation effect, it can inhibit the pathogenic bacteria, maintain the balance of intestinal flora, and promote the absorption of nutrients

LACTOBACILLUS BULGARICUS

Early-stage research suggests the following health conditions that could be prevented: liver disease, common cold, diarrhea caused by antibiotics, atopic dermatitis (eczema) allergic rhinitis ( hay fever), colic, tooth decay, periodontal disease, inflammatory bowel disease and necrotizing enterocolitis.

SACCHAROMICES BOULARDII
Is said to help with the following conditions: Crohn’s disease, diarrhea, irritable bowel syndrome, ulcerative colitis. Saccharomyces boulardii is typically used as a remedy for gastrointestinal problems, acne, canker sores, high cholesterol, urinary tract infections, and yeast infections.

THE UNSUSPECTING HEALTH TO YOUR GUT:

ULTRA-HIGH ACTIVITY – LINKED PROBIOTICS TOLERANCE TEST

BILE SALT RESISTANCE TEST

Bile Salt Tolerance test of AB-Kefir

GASTRIC ACID RESISTANCE TEST

Acid Tolerance Test of AB Kefir at ph 3.0

ULTRA-HIGH ACTIVITY TEN-LINKED PROBIOTICSSTABILITY EXPERIMENT

Storage Stability at 25 °C

VITALITY STABILITY TEST

Storage Stability at 27 °C of AB Kefir

12 BILLION CFU/MUTUALLY BENEFICIAL SYMBIOTIC FERMENTATION- ADJUSTING THE MICROECOLOGY IS MORE EFFECTIVE

FOR LONG LIFEs innovation 12strain bacteria mutualism symbiotic fermentation technology is different from single strain fermentation, providing more stability and value of products.

• Produce abundant metabolic products, such as organic acids, peptides, amino acid, vitamins, trace mineral elements  and other beneficial human factors, improve product nutritional value
• Diversified composite strains,  each lactic acid bacteria has its own different effects to cope with the adverse effects on human body in the environment
• The compound strains grow together, do their duties and help each other, effectively regulating the intestinal microecology
• Processing long-term symbiotic fermentation to produce special natural flavors

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LABORATORY REPORT:

University of Louisville, Department of Pathology

511 South Floyd

Louisville, Kentucky 40202

Vaclav Vetvicka, PhD

Experimental data obtained during previous research projects allowed us to confidently conclude that glucans are highly active immunostimulators. The aim of this study was to evaluate a commercial sample IMMU BOO from FOR LONG LIFE inc. We used two different doses and 14 days oral supplementation. Both cellular and humoral immune response was evaluated, so this project will be able to answer the question whether the sample stimulate one or both branches of the immune system. – healthy lifestyle

Conditions:

1. Balb/c mice will be used, both sexes, age 6-8 weeks
2. Biological activity of glucan will be tested after 14 days of oral feeding (100 or 200 ug/mouse). Declared amount of 25% beta glucan, ie 25 µg at 100 µg and 50 µg at 200 µg
3. Glucans will be compared with a negative control (PBS).
4. Five mice will be used for each experiment.
5. Experiment designed for the total of 11 samples. 

Experiments:

1. Mice were orally given different daily doses of samples for 14 days interval. 
2. Evaluation of phagocytosis in peripheral blood. In order to evaluate the effects of glucan on phagocytosis, synthetic microspheres prepared from 2-hydroxyethylmethacrylate copolymer, were used. Using well established techniques, phagocytic activity of macrophages isolated from peritoneal cavity and monocytes and neutrophils in peripheral blood were tested.
3. Evaluation of the effects of glucan on IL-2 production by splenocytes using a commercial IL-2 ELISA kit. A control group with Concanavalin A was used.
4. Evaluation of the effects of glucan on antibody response. The glucan-fed mice (3 weeks in these experiments) were injected twice (day 0 and day 14) with ovalbumin. On day 21, the mice were killed, serum collected and evaluated for anti-ovalbumin antibodies by ELISA. As control, antigen (ovalbumin) injected sc. with Freud adjuvants, was used.
5. Evaluation of CD4, CD8 and CD19 surface markers by flow cytometry. Cells isolated from the spleen were tested. – healthy lifestyle

Results:

Studies of phagocytosis (both in peritoneal macrophages and blood neutrophils and monocytes) showed that the sample has significant stimulating activity in all types of cells. A small but clear dose-dependence was observed (Figure 1). Similar results were found when we measured IL-2 production (Figure 2). The IL-2 production without any stimulation is usually very low (sometimes even 0), so the observed IL-2 production is statistically significant. 

Next, we focused our attention of possible stimulation of antibody response. Both doses significantly improved antibody response (compared to Ag only), again with clear dose-dependence (Figure 3). The last part of the project was evaluation of possible effects on composition of spleen cells. CD4-positive and CD-8 positive T lymphocytes and B lymphocytes (CD19 cells) were measured by flow cytometry. Small improvements in numbers of B lymphocytes were observed, but these changes were not statistically significant (Figure 4).

Conclusions:

Several conclusions can be reached.

1. Immunostimulatory activity of the test sample to cellular immunity (phagocytosis) was confirmed. There was an increase of 30-40% compared to the control sample without supplementation.
2. The immunostimulatory activity of the test sample on humoral immunity (antibody production and IL-2 secretion) was confirmed. Increase 30 – 40 times compared to standard reaction.
3. An increase in antibody production by 200-300% over control Ag was confirmed.
4. A slight increase of 5-10% in the production of own stem cells was confirmed.

Due to the presence of probiotics, it might be beneficial to further evaluate the possible effects on some problems of the gastrointestinal tract.

_______________________________________

Department of Pathology 

University of Louisville School of Medicine 

By:

Name: Vaclav Vetvicka

Title: Researcher

Date: 28 day of October, 2019

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