IMMUBOO increases immunity in a natural way. Ensure the proper activity of immune cells, eliminate excess burden and and removes old or damaged cells from the body. – healthy lifestyle
LABORATORY REPORT:
University of Louisville, Department of Pathology
511 South Floyd
Louisville, Kentucky 40202
Vaclav Vetvicka, PhD
Experimental data obtained during previous research projects allowed us to confidently conclude that glucans are highly active immunostimulators. The aim of this study was to evaluate a commercial sample IMMU BOO from FOR LONG LIFE inc. We used two different doses and 14 days oral supplementation. Both cellular and humoral immune response was evaluated, so this project will be able to answer the question whether the sample stimulate one or both branches of the immune system. – healthy lifestyle
Conditions:
- Balb/c mice will be used, both sexes, age 6-8 weeks
- Biological activity of glucan will be tested after 14 days of oral feeding (100 or 200 ug/mouse). Declared amount of 25% beta glucan, ie 25 µg at 100 µg and 50 µg at 200 µg
- Glucans will be compared with a negative control (PBS).
- Five mice will be used for each experiment.
- Experiment designed for the total of 11 samples.
Experiments:
- Mice were orally given different daily doses of samples for 14 days interval.
- Evaluation of phagocytosis in peripheral blood. In order to evaluate the effects of glucan on phagocytosis, synthetic microspheres prepared from 2-hydroxyethylmethacrylate copolymer, were used. Using well established techniques, phagocytic activity of macrophages isolated from peritoneal cavity and monocytes and neutrophils in peripheral blood were tested.
- Evaluation of the effects of glucan on IL-2 production by splenocytes using a commercial IL-2 ELISA kit. A control group with Concanavalin A was used.
- Evaluation of the effects of glucan on antibody response. The glucan-fed mice (3 weeks in these experiments) were injected twice (day 0 and day 14) with ovalbumin. On day 21, the mice were killed, serum collected and evaluated for anti-ovalbumin antibodies by ELISA. As control, antigen (ovalbumin) injected sc. with Freud adjuvants, was used.
- Evaluation of CD4, CD8 and CD19 surface markers by flow cytometry. Cells isolated from the spleen were tested. – healthy lifestyle
Results:
Studies of phagocytosis (both in peritoneal macrophages and blood neutrophils and monocytes) showed that the sample has significant stimulating activity in all types of cells. A small but clear dose-dependence was observed (Figure 1). Similar results were found when we measured IL-2 production (Figure 2). The IL-2 production without any stimulation is usually very low (sometimes even 0), so the observed IL-2 production is statistically significant.
Next, we focused our attention of possible stimulation of antibody response. Both doses significantly improved antibody response (compared to Ag only), again with clear dose-dependence (Figure 3). The last part of the project was evaluation of possible effects on composition of spleen cells. CD4-positive and CD-8 positive T lymphocytes and B lymphocytes (CD19 cells) were measured by flow cytometry. Small improvements in numbers of B lymphocytes were observed, but these changes were not statistically significant (Figure 4).
Conclusions:
Several conclusions can be reached.
- Immunostimulatory activity of the test sample to cellular immunity (phagocytosis) was confirmed. There was an increase of 30-40% compared to the control sample without supplementation.
- The immunostimulatory activity of the test sample on humoral immunity (antibody production and IL-2 secretion) was confirmed. Increase 30 – 40 times compared to standard reaction.
- An increase in antibody production by 200-300% over control Ag was confirmed.
- A slight increase of 5-10% in the production of own stem cells was confirmed.
Due to the presence of probiotics, it might be beneficial to further evaluate the possible effects on some problems of the gastrointestinal tract.
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Department of Pathology
University of Louisville School of Medicine
By:
Name: Vaclav Vetvicka
Title: Researcher
Date: 28 day of October, 2019
