Beta-glucans are a type of soluble fiber found in various foods, particularly in the cell walls of certain grains, such as oats and barley, as well as in mushrooms and yeast.

Renowned for their potential health benefits, beta-glucans have gained attention for their positive impact on immune system function and cholesterol levels. These bioactive compounds work by stimulating the immune system, enhancing its ability to defend the body against infections and diseases.

Beta-glucans are increasingly recognized for their contribution to a well-rounded and health-conscious diet. Incorporating sources of beta-glucans into your daily meals can be a smart choice for supporting your immune system and maintaining overall well-being.

Triterpenes are a class of organic compounds widely distributed in nature, known for their diverse range of biological activities and potential health benefits. These naturally occurring compounds are derived from isoprene units and can be found in various plants, fungi, and marine organisms.

Common sources of triterpenes include certain fruits, vegetables, herbs, and medicinal plants. For instance, triterpenoids can be found in abundance in plants like ginseng, licorice, and some mushrooms. While more research is needed to fully understand the extent of their health benefits, triterpenes continue to be an intriguing area of study for their potential therapeutic applications and contribution to natural remedies. Including triterpene-rich foods in your diet may offer a natural way to support your well-being.

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Key ingredients in this proprietary blend include: Crataegus (Hawthorn): Recognized for its cardiovascular benefits, Hawthorn is known to support heart health and promote healthy blood circulation.

Filipendula ulmaria (Meadowsweet): Meadowsweet is valued for its anti-inflammatory and pain-relieving properties and has been traditionally used for various health purposes.

Trifolium pratense (Red Clover): Red Clover contains phytoestrogens and antioxidants, and is often used to support women’s health and address various skin conditions.

Auricularia polytricha (Jelly Ear): This edible mushroom is known for its potential immune-boosting properties and contribution to overall health. Allium fera (Wild Garlic): Wild Garlic has antimicrobial properties and is recognized for its potential benefits in supporting the immune system.

Rosa canina (Dog Rose): Rich in vitamin C and antioxidants, Dog Rose supports immune function and skin health.

Piperis nigrum (Black Pepper): Black Pepper is often included for its ability to enhance the bioavailability of other compounds, promoting better absorption.

This thoughtfully curated blend combines the goodness of medicinal mushrooms with a selection of botanicals known for their various health-promoting properties.

As with any supplement, it is advisable to consult with a healthcare professional before incorporating it into your wellness routine.

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Food grade diatomaceous earth COLOFLUSH is an ancient substance and a true modern natural treasure – a safer and simpler solution to our daily needs.

It is composed of the fossilized siliceous skeletons or remains of diatoms. Diatoms are a major group of microscopic algae or unicellular aquatic plants and are among the most common types of phytoplankton. That once flourished as a form of golden brown aquatic algae or plankton that lived in fresh water lakes as a food source for all kinds of aquatic creatures. 

Our COLOFLUSH is cleaned and sterilized in our unique production and combined with baking soda to an alkaline diatomaceous earth for human consumption and contains NO additives. It is a fine, off-white, 10-mim microfine powder that disperses easily in liquid. Contains an average of 96% silica and less than 0.5% crystalline silica. 

The many amazing benefits of FOR LONG LIFE food grade diatomaceous earth:

COLOFLUSH ADVANCED FORMULATED POWDER PLEASE DO NOT CONFUSE WITH INDUSTRIAL SWIMMING POOL SILICATING AGENT THAT HAS BEEN HEAT AND CHEMICALLY TREATED AND INTENDED FOR POOLS AND FISH FILTERS ONLY.

IT IS TOXIC TO LIVING ENTITIES.

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Medical research verified that Bifidobacterium longum has the effect of regulating human intestines and improving health – healthy lifestyle

STREPTOCOCCUS THERMOPHILUS

Can reduce intestinal ph, promote bowels movement to prevent colonization of pathogenic bacteria, secrete bacteriocin to inhibit the growth of pathogenic bacteria

LACTOBACILLUS PARACASEI

It helps promote the balance of microbial flora in the body and stimulate specific and non-specific immune mechanisms, which can prevent certain diseases, enhance physical fitness, and delay aging – healthy lifestyle

LACTOBACILLUS REUTERI

It has strong adhesion to intestinal mucosa to improve the distribution of intestinal flora, antagonize the colonization of harmful bacteria, and avoid suffering from intestinal diseases

LACTOBACILLUS CASEI

It treats intestinal flora disorders and enhances intestinal permeability, improves immunity

LACTOBACILLUS ACIDOPHILUS

Adjusts the balance of the intestinal flora,  helps secrete avidin, and inhibits the proliferation of intestinal microbes

LACTOBACILLUS RHAMNOSUS

Works as a biological barrier of the human intestinal mucosa, regulates intestinal flora, and enhances the body’s immunity

LACTOBACILLUS FERMENTUM

Improves the intestinal environment, regulates intestinal flora, improves immunity

LACTOBACILLUS HELVETICUS

It can adjust the balance of intestinal micro flora and treat diarrhea

LACTOBACILLUS PLANTARUM

With a certain immune regulation effect, it can inhibit the pathogenic bacteria, maintain the balance of intestinal flora, and promote the absorption of nutrients

LACTOBACILLUS BULGARICUS

Early-stage research suggests the following health conditions that could be prevented: liver disease, common cold, diarrhea caused by antibiotics, atopic dermatitis (eczema) allergic rhinitis ( hay fever), colic, tooth decay, periodontal disease, inflammatory bowel disease and necrotizing enterocolitis.

SACCHAROMICES BOULARDII
Is said to help with the following conditions: Crohn’s disease, diarrhea, irritable bowel syndrome, ulcerative colitis. Saccharomyces boulardii is typically used as a remedy for gastrointestinal problems, acne, canker sores, high cholesterol, urinary tract infections, and yeast infections.

THE UNSUSPECTING HEALTH TO YOUR GUT:

ULTRA-HIGH ACTIVITY – LINKED PROBIOTICS TOLERANCE TEST

BILE SALT RESISTANCE TEST

Bile Salt Tolerance test of AB-Kefir

GASTRIC ACID RESISTANCE TEST

Acid Tolerance Test of AB Kefir at ph 3.0

ULTRA-HIGH ACTIVITY TEN-LINKED PROBIOTICSSTABILITY EXPERIMENT

Storage Stability at 25 °C

VITALITY STABILITY TEST

Storage Stability at 27 °C of AB Kefir

12 BILLION CFU/MUTUALLY BENEFICIAL SYMBIOTIC FERMENTATION- ADJUSTING THE MICROECOLOGY IS MORE EFFECTIVE

FOR LONG LIFEs innovation 12strain bacteria mutualism symbiotic fermentation technology is different from single strain fermentation, providing more stability and value of products.

• Produce abundant metabolic products, such as organic acids, peptides, amino acid, vitamins, trace mineral elements  and other beneficial human factors, improve product nutritional value
• Diversified composite strains,  each lactic acid bacteria has its own different effects to cope with the adverse effects on human body in the environment
• The compound strains grow together, do their duties and help each other, effectively regulating the intestinal microecology
• Processing long-term symbiotic fermentation to produce special natural flavors

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Serrapeptase is used to treat inflammation, reduce pain and swelling, speed up the healing process and support the immune system. It has a cleansing effect, helps to bind heavy metals, and helps to eliminate toxins from the body. Its positive effects are clinically confirmed in the treatment of a wide range of health problems:

• Cardiovascular diseases
• High blood pressure
• Type II diabetes and diabetic neuropathy
• Osteoporosis
• Multiple sclerosis
• Migraine and headaches
• Cystic breast fibrosis 
• Joint inflammation, arthritis, rheumatoid arthritis 
• Inflammation of the upper and lower respiratory tract
• Inflammation of various kinds
• Intestinal diseases
• Asthma
• Injuries and sports injuries
• Cleansing the body of toxins

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Among other things, it can speed up the healing process of bruises. This is evidenced by less swelling in the affected area. The natural substances used can increase blood microcirculation in the affected area and thus accelerate the active breakdown of free oxygen radicals and toxins. At the same time, regeneration and reconstruction processes in the affected cells can be accelerated. This could bring a quick feeling of refreshment and loosening of the affected area.

Instructions for use: The gel is applied to tired or sore areas of the body and gently massaged in. The massage is always performed towards the heart to facilitate blood flow. Apply as needed before or after any physical activity or for general well-being. Not more than 4-6 times a day.

It absorbs well, does not leave an oily or sticky film on the skin and does not irritate the skin. To achieve the maximum effect, it is advisable to apply the gel to the affected area and cover it with a cling film and leave it to take effect for a longer time (e.g., overnight). – healthy lifestyle

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LABORATORY REPORT:

University of Louisville, Department of Pathology

511 South Floyd

Louisville, Kentucky 40202

Vaclav Vetvicka, PhD

Experimental data obtained during previous research projects allowed us to confidently conclude that glucans are highly active immunostimulators. The aim of this study was to evaluate a commercial sample IMMU BOO from FOR LONG LIFE inc. We used two different doses and 14 days oral supplementation. Both cellular and humoral immune response was evaluated, so this project will be able to answer the question whether the sample stimulate one or both branches of the immune system. – healthy lifestyle

Conditions:

1. Balb/c mice will be used, both sexes, age 6-8 weeks
2. Biological activity of glucan will be tested after 14 days of oral feeding (100 or 200 ug/mouse). Declared amount of 25% beta glucan, ie 25 µg at 100 µg and 50 µg at 200 µg
3. Glucans will be compared with a negative control (PBS).
4. Five mice will be used for each experiment.
5. Experiment designed for the total of 11 samples. 

Experiments:

1. Mice were orally given different daily doses of samples for 14 days interval. 
2. Evaluation of phagocytosis in peripheral blood. In order to evaluate the effects of glucan on phagocytosis, synthetic microspheres prepared from 2-hydroxyethylmethacrylate copolymer, were used. Using well established techniques, phagocytic activity of macrophages isolated from peritoneal cavity and monocytes and neutrophils in peripheral blood were tested.
3. Evaluation of the effects of glucan on IL-2 production by splenocytes using a commercial IL-2 ELISA kit. A control group with Concanavalin A was used.
4. Evaluation of the effects of glucan on antibody response. The glucan-fed mice (3 weeks in these experiments) were injected twice (day 0 and day 14) with ovalbumin. On day 21, the mice were killed, serum collected and evaluated for anti-ovalbumin antibodies by ELISA. As control, antigen (ovalbumin) injected sc. with Freud adjuvants, was used.
5. Evaluation of CD4, CD8 and CD19 surface markers by flow cytometry. Cells isolated from the spleen were tested. – healthy lifestyle

Results:

Studies of phagocytosis (both in peritoneal macrophages and blood neutrophils and monocytes) showed that the sample has significant stimulating activity in all types of cells. A small but clear dose-dependence was observed (Figure 1). Similar results were found when we measured IL-2 production (Figure 2). The IL-2 production without any stimulation is usually very low (sometimes even 0), so the observed IL-2 production is statistically significant. 

Next, we focused our attention of possible stimulation of antibody response. Both doses significantly improved antibody response (compared to Ag only), again with clear dose-dependence (Figure 3). The last part of the project was evaluation of possible effects on composition of spleen cells. CD4-positive and CD-8 positive T lymphocytes and B lymphocytes (CD19 cells) were measured by flow cytometry. Small improvements in numbers of B lymphocytes were observed, but these changes were not statistically significant (Figure 4).

Conclusions:

Several conclusions can be reached.

1. Immunostimulatory activity of the test sample to cellular immunity (phagocytosis) was confirmed. There was an increase of 30-40% compared to the control sample without supplementation.
2. The immunostimulatory activity of the test sample on humoral immunity (antibody production and IL-2 secretion) was confirmed. Increase 30 – 40 times compared to standard reaction.
3. An increase in antibody production by 200-300% over control Ag was confirmed.
4. A slight increase of 5-10% in the production of own stem cells was confirmed.

Due to the presence of probiotics, it might be beneficial to further evaluate the possible effects on some problems of the gastrointestinal tract.

_______________________________________

Department of Pathology 

University of Louisville School of Medicine 

By:

Name: Vaclav Vetvicka

Title: Researcher

Date: 28 day of October, 2019

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